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7.5 Calibration and quantification

IC Net 2.0

121

7.5.3 External standard calibration

External standard calibration (absolute calibration) is a key way of

calibration. Basically this procedure calculates the dependence

of injected component's quantity versus area (or height) of the

corresponding peak. This dependence is showed as a calibration

plot with injected quantity along the Y axis and peak area (or

height) along the X axis. The injected quantity Q

is calculated as

product of component's concentration C

to the reduced injected

sample volume V':

= C

• V'

The calibration function W

(R) for each component is calculated

using the RSD method.

(R) = RSD(Q

, R

) = RSD((C

• V'), R

)

When quantification procedure is performed, a concentration C

(absolute concentration of a component in the sample) is deter-

mined as a ratio of the computed quantity W

) to the corrected

volume of sample injected V':

= W

) / V'

7.5.4 Component table

IC NET / Method / Calibration / Components

If the component table is opened, the chromatogram window is

split into two parts. The upper one shows the chromatogram, the

component table appears in the bottom part of the screen. When

moving into the component table, a special cursor in the upper

part of the window jumps to the peak corresponding to the cur-

rent component.

The component table stores the following information on the

components to be analyzed:

1 2 ... 124 125 126 127 128 129 130 131 132 133 134 ... 195 196

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Metrohm IC Net 2.0 Manual de usuario Pagina 129